Anti-SCARA5 Polyclonal Antibody (HV465014)

Name: Anti-SCARA5 Polyclonal Antibody
Brand: abinScience
SKU: HV465014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV465014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SCARA5 (Gln118-His495).

Target

SCARA5, Scavenger receptor hlg, Scavenger receptor class A member 5

Purification

Purified by antigen affinity column.

Accession

Q6ZMJ2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Scavenger receptor class A member 5 (SCARA5) is a ~53 kDa protein. Ferritin receptor that mediates non-transferrin-dependent delivery of iron. Mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Preferentially binds ferritin light chain (FTL) compared to heavy chain (FTH1).

Note

For research use only.

Images

Western Blot

Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SCARA5 antibody (HV465014) at 1 μg/ml.

Lane 1: Recombinant protein

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (TF690314) at 0.1 μg/mL.

Predict MW: 43 kDa
Observed MW: 43 kDa
Western Blot

Rat Muscle lysates were subjected to SDS PAGE followed by western blot with SCARA5 antibody (HV465014) at 1 μg/ml.

Lane 1: Rat Muscle lysates

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (TF690314) at 0.1 μg/mL.

Predict MW: 43 kDa
Observed MW: 43 kDa

References

Datasheet

Anti-SCARA5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SCARA5 Polyclonal Antibody (HV465014)

Overview

Images

References

Datasheet

SDS

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