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Anti-SEBOV VP40 Polyclonal Antibody (VK783024)

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Overview
Catalog No.VK783024
Description
Anti-SEBOV VP40 Polyclonal Antibody (VK783024) is a rabbit polyclonal antibody detecting VP40 in ELISA, IHC, WB. Suitable for Sudan ebolavirus and Reston ebolavirus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivitySudan ebolavirus (SEBOV) (Sudan Ebola virus), Reston ebolavirus (REBOV) (Reston Ebola virus)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant SEBOV VP40 (Met44-Gly294).
Target matrix protein, VP40
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q8JPX9, Q5XX06
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Matrix protein VP40 is a ~35 kDa protein. Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding. May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis).

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-SEBOV VP40 Polyclonal Antibody

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