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Anti-SEMA3C Polyclonal Antibody (HV628014)

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Anti-SEMA3C Polyclonal Antibody
Anti-SEMA3C Polyclonal Antibody
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Overview
Catalog No.HV628014
Description
Anti-SEMA3C Polyclonal Antibody (HV628014) is a rabbit polyclonal antibody detecting SEMA3C in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Chicken, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SEMA3C (Arg28-Asp150).
Target SEMA3C, Semaphorin-E, Semaphorin-3C, SEMAE, Sema E
Purification Purified by antigen affinity column.
Accession Q99985
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Semaphorin-3C (SEMA3C) is a ~85 kDa protein. Binds to plexin family members and plays an important role in the regulation of developmental processes. Required for normal cardiovascular development during embryogenesis. Functions as attractant for growing axons, and thereby plays an important role in axon growth and axon guidance.

Note For research use only.
Images
  • Anti-SEMA3C Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-SEMA3C polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Raw264.7 cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: PC12 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: Mouse lung lysate
    Lane 6: Mouse small intestine lysate
    Lane 7: Mouse heart lysate
    Lane 8: Rat lung lysate
    Lane 9: Rat small intestine lysate
    Lane 10: Rat heart lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-SEMA3C Polyclonal Antibody

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