Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody (VK022014)

Name: Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody
Brand: abinScience
SKU: VK022014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

VK022014

Description

Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody (VK022014) is a rabbit polyclonal antibody detecting Capsid protein in WB, IHC, ELISA. Suitable for O'nyong-nyong virus, Sagiyama virus, Chikungunya virus, Ross river virus, and Middelburg virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Semliki Forest virus (SFV), O'nyong'nyong virus (ONNV), Chikungunya virus (CHIKV), Ross River virus (RRV), Getah virus (GETV), Mayaro virus (MAYV)

Applications

WB,IHC,ELISA

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Semliki forest virus/SFV Capsid (Cys119-Trp267).

Target

Structural polyprotein, p130, Capsid protein, EC:3.4.21.90, Coat protein, C

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P03315, P0DJZ6

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Structural polyprotein (Capsid) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Roussel, A. et al. (2006) Structure (London, England : 1993) 14, 75-86. PMID: 16407067

2. Melancon, P. et al. (1987) Journal of virology 61, 1301-9. PMID: 3553612

3. Skoging, U. et al. (1998) Journal of molecular biology 279, 865-72. PMID: 9642067

4. Ulmanen, I. et al. (1979) Virology 99, 265-76. PMID: 516447

5. Vonderheit, A. et al. (2005) PLoS biology 3, e233. PMID: 15954801

6. Singh, I. et al. (1992) Journal of virology 66, 7049-58. PMID: 1433506

Note

For research use only.

Images

Western blot

Lane 1: recombinant Semliki forest virus/SFV Capsid (Catalog No: VK022012)

References

Datasheet

Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody (VK022014)

Overview

Images

References

Datasheet

SDS