Anti-SF3A1 Polyclonal Antibody (HB446014)

Name: Anti-SF3A1 Polyclonal Antibody
Brand: abinScience
SKU: HB446014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB446014

Description

Anti-SF3A1 Polyclonal Antibody (HB446014) is a rabbit polyclonal antibody detecting Splicing factor 3A subunit 1; SF3a120; Spliceosome-associated protein 114; SAP 114; SF3A1; SAP114 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human SF3A1 (Ser690-Lys793).

Target

Splicing factor 3A subunit 1; SF3a120; Spliceosome-associated protein 114; SAP 114; SF3A1; SAP114

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

A2VDN6, Q15459, Q8K4Z5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Splicing factor 3A subunit 1 (SF3A1) is a ~88 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-SF3A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HL-60 cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: Lncap cell lysate
Lane 5: Hela cell lysate
Lane 6: HepG2 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse brain lysate
Lane 9: Mouse lung lysate
Lane 10: Mouse testis lysate
Lane11: Rat brain lysate
Lane12: Rat lung lysate

References

Datasheet

Anti-SF3A1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SF3A1 Polyclonal Antibody (HB446014)

Overview

Images

References

Datasheet

SDS

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