Anti-SHMT2 Polyclonal Antibody (HW418014)

Name: Anti-SHMT2 Polyclonal Antibody
Brand: abinScience
SKU: HW418014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HW418014

Description

Anti-SHMT2 Polyclonal Antibody (HW418014) is a rabbit polyclonal antibody detecting SHMT2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SHMT2 (Asn30-His504).

Target

SHMT2, Serine hydroxymethyltransferase, mitochondrial, SHMT, Glycine hydroxymethyltransferase, Serine methylase

Purification

Purified by antigen affinity column.

Accession

P34897

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine hydroxymethyltransferase, mitochondrial (SHMT2) is a ~55 kDa protein. Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis. Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA. Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs. Associates with mitochondrial DNA.

1. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985

2. Giardina, G. et al. (2015) The FEBS journal 282, 1225-41. PMID: 25619277

3. Morscher, RJ. et al. (2018) Nature 554, 128-132. PMID: 29364879

4. García-Cazorla, À. et al. (2020) Acta neuropathologica 140, 971-975. PMID: 33015733

5. Anderson, DD. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15163-8. PMID: 21876188

6. Minton, DR. et al. (2018) Molecular cell 69, 610-621.e5. PMID: 29452640

7. Bogenhagen, DF. et al. (2008) The Journal of biological chemistry 283, 3665-3675. PMID: 18063578

Note

For research use only.

Images

References

Datasheet

Anti-SHMT2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SHMT2 Polyclonal Antibody (HW418014)

Overview

Images

References

Datasheet

SDS

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