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Anti-SHPRH Polyclonal Antibody (HB530014)

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Overview
Catalog No.HB530014
Description
Anti-SHPRH Polyclonal Antibody (HB530014) is a rabbit polyclonal antibody detecting E3 ubiquitin-protein ligase SHPRH; 2.3.2.27; 3.6.4.-; RING-type E3 ubiquitin transferase SHPRH; SNF2 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human SHPRH (Glu1381-Glu1683).
Target E3 ubiquitin-protein ligase SHPRH; 2.3.2.27; 3.6.4.-; RING-type E3 ubiquitin transferase SHPRH; SNF2, histone-linker, PHD and RING finger domain-containing helicase; SHPRH; KIAA2023
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q149N8, Q7TPQ3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

E3 ubiquitin-protein ligase SHPRH is a ~193 kDa protein. E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.

Note For research use only
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Formula
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Anti-SHPRH Polyclonal Antibody

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