Anti-SIV Integrase Polyclonal Antibody (VK100024)

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Datasheet

Overview

Catalog No.

VK100024

Description

Anti-SIV Integrase Polyclonal Antibody (VK100024) is a rabbit polyclonal antibody detecting Integrase in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Simian immunodeficiency virus agm.vervet

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant SIV Integrase (Phe1174-Ser1470).

Target

Gag-Pol polyprotein, Pr160Gag-Pol, Matrix protein p17, MA, Capsid protein p24, CA, Nucleocapsid protein p7, NC, p6-pol, p6*, Protease, 3.4.23.16, PR, Retropepsin, Reverse transcriptase/ribonuclease H, 2.7.7.49, 2.7.7.7, 3.1.26.13, Exoribonuclease H, 3.1.13.2, p66 RT, p51 RT, p15, Integrase, IN, 2.7.7.-, 3.1.-.-, gag-pol

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P27980, P27973

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Gag-Pol polyprotein (Integrase) is a ~166 kDa protein. Gag-Pol polyprotein and Gag polyprotein may regulate their own translation, by the binding genomic RNA in the 5'-UTR. At low concentration, Gag-Pol and Gag would promote translation, whereas at high concentration, the polyproteins encapsidate genomic RNA and then shut off translation.

Note

For research use only

Images

References

Datasheet

Anti-SIV Integrase Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SIV Integrase Polyclonal Antibody (VK100024)

Overview

Images

References

Datasheet

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