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Anti-SLBP Polyclonal Antibody (HA797014)

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Overview
Catalog No.HA797014
Description
Anti-SLBP Polyclonal Antibody (HA797014) is a rabbit polyclonal antibody detecting Histone RNA hairpin-binding protein; Histone stem-loop-binding protein; SLBP; HBP in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human SLBP (Cys72-Asp198).
Target Histone RNA hairpin-binding protein; Histone stem-loop-binding protein; SLBP; HBP
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q14493, P97440
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Histone RNA hairpin-binding protein (SLBP) is a ~31 kDa protein. RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis.

1. Zheng, L. et al. (2003) Molecular and cellular biology 23, 1590-601. PMID: 12588979
2. Sullivan, KD. et al. (2009) RNA (New York, N.Y.) 15, 459-72. PMID: 19155325
3. Wang, ZF. et al. (1996) Genes & development 10, 3028-40. PMID: 8957003
4. Martin, F. et al. (1997) The EMBO journal 16, 769-78. PMID: 9049306
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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