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Anti-SLC12A3 Polyclonal Antibody (HX864014)

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Overview
Catalog No.HX864014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SLC12A3 (Glu3-Cys146).
Target TSC, SLC12A3, NCC, Solute carrier family 12 member 3, Na-Cl cotransporter, Thiazide-sensitive sodium-chloride cotransporter, Na-Cl symporter
Purification Purified by antigen affinity column.
Accession P55017
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Solute carrier family 12 member 3 (SLC12A3) is a ~113 kDa protein. Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules. Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1.

1. Richardson, C. et al. (2008) Journal of cell science 121, 675-84. PMID: 18270262
2. Cruz-Rangel, S. et al. (2011) American journal of physiology. Cell physiology 301, C601-8. PMID: 21613606
3. Glaudemans, B. et al. (2012) European journal of human genetics : EJHG 20, 263-70. PMID: 22009145
4. Nan, J. et al. (2022) Science advances 8, eadd7176. PMID: 36351028
5. Fan, M. et al. (2023) Nature 614, 788-793. PMID: 36792826
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-SLC12A3 Polyclonal Antibody

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