Anti-SLC40A1 Polyclonal Antibody (HV370014)

Name: Anti-SLC40A1 Polyclonal Antibody
Brand: abinScience
SKU: HV370014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV370014

Description

Anti-SLC40A1 Polyclonal Antibody (HV370014) is a rabbit polyclonal antibody detecting SLC40A1 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SLC40A1 (Trp127-Val321).

Target

Solute carrier family 40 member 1, SLC11A3, FPN1, SLC40A1, Iron-regulated transporter 1, IREG1, Ferroportin-1

Purification

Purified by antigen affinity column.

Accession

Q9NP59

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ferroportin (FPN1/SLC40A1) is a ~62 kDa protein. Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes. When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma.

1. Schimanski, LM. et al. (2005) Blood 105, 4096-102. PMID: 15692071

2. Madejczyk, MS. et al. (2012) Biochimica et biophysica acta 1818, 651-7. PMID: 22178646

3. Qiao, B. et al. (2012) Cell metabolism 15, 918-24. PMID: 22682227

4. Mitchell, CJ. et al. (2014) American journal of physiology. Cell physiology 306, C450-9. PMID: 24304836

5. Aschemeyer, S. et al. (2018) Blood 131, 899-910. PMID: 29237594

6. Zhang, DL. et al. (2018) Science (New York, N.Y.) 359, 1520-1523. PMID: 29599243

7. Choi, EK. et al. (2019) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33, 2228-2240. PMID: 30247984

8. Billesbølle, CB. et al. (2020) Nature 586, 807-811. PMID: 32814342

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SLC40A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: Caco2 cell lysate
Lane 4: Rat liver lysate