Anti-SMAD5 Polyclonal Antibody (HV175014)

Name: Anti-SMAD5 Polyclonal Antibody
Brand: abinScience
SKU: HV175014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV175014

Description

Anti-SMAD5 Polyclonal Antibody (HV175014) is a rabbit polyclonal antibody detecting SMAD5 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Coturnix japonica.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SMAD5 (Gly210-Val464).

Target

SMAD 5, Mothers against decapentaplegic homolog 5, SMAD5, Mothers against DPP homolog 5, Smad5, SMAD family member 5, MAD homolog 5, JV5-1, MADH5, hSmad5

Purification

Purified by antigen affinity column.

Accession

Q99717

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

SMAD family member 5 (SMAD5) is a ~52 kDa protein. Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis.

1. Fuchs, O. et al. (2002) Blood cells, molecules & diseases 28, 221-33. PMID: 12064918

2. Haag, J. et al. (2006) Experimental cell research 312, 1153-63. PMID: 16516194

3. Nishimura, R. et al. (1998) The Journal of biological chemistry 273, 1872-9. PMID: 9442019

4. Ruiz, L. et al. (2021) Computational and structural biotechnology journal 19, 632-646. PMID: 33510867

5. Fang, Y. et al. (2017) Cell research 27, 1083-1099. PMID: 28675158

Note

For research use only.

Images

References

Datasheet

Anti-SMAD5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SMAD5 Polyclonal Antibody (HV175014)

Overview

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References

Datasheet

SDS

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