Anti-SMARCD3 Polyclonal Antibody (HB357014)

Name: Anti-SMARCD3 Polyclonal Antibody
Brand: abinScience
SKU: HB357014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HB357014

Description

Anti-SMARCD3 Polyclonal Antibody (HB357014) is a rabbit polyclonal antibody detecting SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3; 60 kDa BRG-1/Brm-associated factor subunit C; BRG1-associated factor 60C; BAF60C; SMARCD3; BAF60C in ELISA, IHC, WB. Suitable for Mouse and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human SMARCD3 (Arg149-Pro367).

Target

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3; 60 kDa BRG-1/Brm-associated factor subunit C; BRG1-associated factor 60C; BAF60C; SMARCD3; BAF60C

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q6P9Z1, Q6STE5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (SMARCD3) is a ~54 kDa protein. Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state.

1. Euskirchen, G. et al. (2012) The Journal of biological chemistry 287, 30897-905. PMID: 22952240

2. Kadoch, C. et al. (2015) Science advances 1, e1500447. PMID: 26601204

3. Lessard, J. et al. (2007) Neuron 55, 201-15. PMID: 17640523

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-SMARCD3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: BxPC-3 cell lysate
Lane 2: Hela cell lysate
Lane 3: U87 cell lysate
Lane 4: 293T cell lysate
Lane 5: K562 cell lysate
Lane 6: Jurkat cell lysate

References

Datasheet

Anti-SMARCD3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-SMARCD3 Polyclonal Antibody (HB357014)

Overview

Images

References

Datasheet

SDS

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