Anti-SMPD1 Polyclonal Antibody (HB267014)

Name: Anti-SMPD1 Polyclonal Antibody
Brand: abinScience
SKU: HB267014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HB267014

Description

Anti-SMPD1 Polyclonal Antibody (HB267014) is a rabbit polyclonal antibody detecting SMPD1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SMPD1 (Gly319-Gly579).

Target

aSMase, SMPD1, Acid sphingomyelinase, Sphingomyelin phosphodiesterase, ASM

Purification

Purified by antigen affinity column.

Accession

P17405

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Sphingomyelin phosphodiesterase (SMPD1) is a ~69 kDa protein. Converts sphingomyelin to ceramide. Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly. However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form.

1. Grassmé, H. et al. (2003) Nature medicine 9, 322-30. PMID: 12563314

2. Schuchman, EH. et al. (1991) The Journal of biological chemistry 266, 8531-9. PMID: 1840600

3. Jones, I. et al. (2008) Molecular genetics and metabolism 95, 152-62. PMID: 18815062

4. Oninla, VO. et al. (2014) Journal of lipid research 55, 2606-19. PMID: 25339683

5. Acuña, M. et al. (2016) European journal of human genetics : EJHG 24, 208-13. PMID: 25920558

6. Acuña, M. et al. (2016) Biochemical and biophysical research communications 479, 496-501. PMID: 27659707

7. Carpinteiro, A. et al. (2020) Cell reports. Medicine 1, 100142. PMID: 33163980

8. Jenkins, RW. et al. (2010) The Journal of biological chemistry 285, 35706-18. PMID: 20807762

9. Jenkins, RW. et al. (2011) The Journal of biological chemistry 286, 3777-88. PMID: 21098024

10. Schissel, SL. et al. (1998) The Journal of biological chemistry 273, 18250-9. PMID: 9660788

Note

For research use only.

Images

References

Datasheet

Anti-SMPD1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SMPD1 Polyclonal Antibody (HB267014)

Overview

Images

References

Datasheet

SDS

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