Anti-SNAI1 Polyclonal Antibody (HF898014)

Name: Anti-SNAI1 Polyclonal Antibody
Brand: abinScience
SKU: HF898014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HF898014

Description

Anti-SNAI1 Polyclonal Antibody (HF898014) is a rabbit polyclonal antibody detecting SNAI1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SNAI1 (Met1-Arg264).

Target

Protein sna, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, SNAH

Purification

Purified by antigen affinity column.

Accession

O95863

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Zinc finger protein SNAI1 is a ~29 kDa protein. Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription. SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits.

1. Batlle, E. et al. (2000) Nature cell biology 2, 84-9. PMID: 10655587

2. Yook, JI. et al. (2005) The Journal of biological chemistry 280, 11740-8. PMID: 15647282

3. Lin, Y. et al. (2010) The EMBO journal 29, 1803-16. PMID: 20389281

4. Lin, T. et al. (2010) Oncogene 29, 4896-904. PMID: 20562920

5. Zhang, K. et al. (2012) The EMBO journal 31, 29-43. PMID: 21952048

6. Jin, Y. et al. (2015) Cancer letters 362, 70-82. PMID: 25827072

8. Tortorici, M. et al. (2013) ACS chemical biology 8, 1677-82. PMID: 23721412

9. Peinado, H. et al. (2005) The EMBO journal 24, 3446-58. PMID: 16096638

10. Hu, CT. et al. (2010) The FEBS journal 277, 1202-18. PMID: 20121949

Note

For research use only.

Images

References

Datasheet

Anti-SNAI1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-SNAI1 Polyclonal Antibody (HF898014)

Overview

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References

Datasheet

SDS

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