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Anti-SOD2 Polyclonal Antibody (HY370014)

Anti-SOD2 Polyclonal Antibody
Anti-SOD2 Polyclonal Antibody
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Overview
Catalog No.HY370014
Description
Anti-SOD2 Polyclonal Antibody (HY370014) is a rabbit polyclonal antibody detecting SOD2 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Bovine, and Callithrix jacchus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SOD2 (Lys25-Lys222).
Target Superoxide dismutase [Mn], mitochondrial, SOD2
Purification Purified by antigen affinity column.
Accession P04179
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Superoxide dismutase [Mn], mitochondrial (SOD2) is a ~24 kDa protein. Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Note For research use only.
Images
  • Anti-SOD2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-SOD2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: DU145 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: U2OS cell lysate
    Lane 5: NIH3T3 cell lysate
    Lane 6: SH-SY5Y cell lysate
    Lane 7: Mouse brain lysate
    Lane 8: Mouse heart lysate
    Lane 9: Rat brain lysate
    Lane 10: Rat heart lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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