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Anti-SORD Polyclonal Antibody (HX841014)

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Overview
Catalog No.HX841014
Description
Anti-SORD Polyclonal Antibody (HX841014) is a rabbit polyclonal antibody detecting SORD in ELISA, IHC, WB. Suitable for Human, Bovine, Macaca fascicularis, Ovis aries, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SORD (Ala98-Gln355).
Target (R, R)-butanediol dehydrogenase, RDH, Polyol dehydrogenase, Sorbitol dehydrogenase, Ribitol dehydrogenase, SORD, Xylitol dehydrogenase, XDH, L-iditol 2-dehydrogenase, SDH
Purification Purified by antigen affinity column.
Accession Q00796
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Sorbitol dehydrogenase (SORD) is a ~38 kDa protein. Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively. Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia. May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility.

1. Maret, W. et al. (1988) Biochemistry 27, 1622-8. PMID: 3365415
2. Pauly, TA. et al. (2003) Structure (London, England : 1993) 11, 1071-85. PMID: 12962626
3. Safi, SZ. et al. (2014) BioMed research international 2014, 801269. PMID: 25105142
4. Dewanjee, S. et al. (2018) European journal of pharmacology 833, 472-523. PMID: 29966615
5. Frenette, G. et al. (2006) Journal of andrology 27, 233-9. PMID: 16278369
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-SORD Polyclonal Antibody

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