Anti-SP1 Polyclonal Antibody (HY593014)

Name: Anti-SP1 Polyclonal Antibody
Brand: abinScience
SKU: HY593014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY593014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SP1 (Leu260-Ala591).

Target

SP1, TSFP1, Transcription factor Sp1

Purification

Purified by antigen affinity column.

Accession

P08047

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription factor Sp1 is a ~80 kDa protein. Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Also binds the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage.

1. Ding, H. et al. (1999) The Journal of biological chemistry 274, 19573-80. PMID: 10391891

2. Yang, X. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 6611-6. PMID: 11371615

3. Milanini-Mongiat, J. et al. (2002) The Journal of biological chemistry 277, 20631-9. PMID: 11904305

4. Bonello, MR. et al. (2004) The Journal of biological chemistry 279, 2377-82. PMID: 14593115

5. Hsu, MC. et al. (2006) The Journal of biological chemistry 281, 4718-25. PMID: 16377629

6. Hung, JJ. et al. (2006) Molecular and cellular biology 26, 1770-85. PMID: 16478997

7. Zhang, Y. et al. (2006) Molecular and cellular biology 26, 6748-61. PMID: 16943418

8. Vicart, A. et al. (2006) Journal of molecular biology 364, 897-908. PMID: 17049555

9. Olofsson, BA. et al. (2007) Molecular cancer research : MCR 5, 1319-30. PMID: 18171990

10. Chuang, JY. et al. (2008) Molecular biology of the cell 19, 1139-51. PMID: 18199680

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate
Lane 3: HepG2 cell lysate