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Anti-SPINT2 Polyclonal Antibody (HT052014)

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Overview
Catalog No.HT052014
Description
Anti-SPINT2 Polyclonal Antibody (HT052014) is a rabbit polyclonal antibody detecting SPINT2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SPINT2 (Ala28-Lys197).
Target SPINT2, Placental bikunin, Hepatocyte growth factor activator inhibitor type 2, Kunitz-type protease inhibitor 2, HAI-2, HAI2, KOP
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O43291
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Kunitz-type protease inhibitor 2 (SPINT2) is a ~28 kDa protein. Inhibitor of HGFAC. Also inhibits plasmin, and plasma and tissue kallikrein. Inhibits serine protease activity of TMPRSS13. Inhibits serine protease activity of ST14/matriptase and PRSS8/prostasin in vitro.

1. Kawaguchi, T. et al. (1997) The Journal of biological chemistry 272, 27558-64. PMID: 9346890
2. Marlor, CW. et al. (1997) The Journal of biological chemistry 272, 12202-8. PMID: 9115294
3. Hashimoto, T. et al. (2010) The FEBS journal 277, 4888-900. PMID: 20977675
4. Murray, AS. et al. (2017) The Journal of biological chemistry 292, 14867-14884. PMID: 28710277
5. Holt-Danborg, L. et al. (2019) Human molecular genetics 28, 828-841. PMID: 30445423
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-SPINT2 Polyclonal Antibody

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