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Anti-SPON2 Polyclonal Antibody (HV224014)

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Anti-SPON2 Polyclonal Antibody
Anti-SPON2 Polyclonal Antibody
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Overview
Catalog No.HV224014
Description
Anti-SPON2 Polyclonal Antibody (HV224014) is a rabbit polyclonal antibody detecting SPON2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SPON2 (Ser119-Val331).
Target DIL-1, SPON2, Mindin, DIL1, Differentially expressed in cancerous and non-cancerous lung cells 1, Spondin-2
Purification Purified by antigen affinity column.
Accession Q9BUD6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Spondin-2 (SPON2) is a ~35 kDa protein. Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).

Note For research use only.
Images
  • Anti-SPON2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-SPON2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Lncap cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-SPON2 Polyclonal Antibody

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