Anti-SRSF3 Polyclonal Antibody (HB340014)

Name: Anti-SRSF3 Polyclonal Antibody
Brand: abinScience
SKU: HB340014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB340014

Description

Anti-SRSF3 Polyclonal Antibody (HB340014) is a rabbit polyclonal antibody detecting arginine/serine-rich 3; SRSF3; SFRS3; SRP20 in ELISA, IHC, WB. Suitable for Mouse, Human, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human SRSF3 (His2-Glu84).

Target

Serine/arginine-rich splicing factor 3; Pre-mRNA-splicing factor SRP20; Splicing factor, arginine/serine-rich 3; SRSF3; SFRS3; SRP20

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P84104, P84103, Q3SZR8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/arginine-rich splicing factor 3 (SRSF3) is a ~19 kDa protein. Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons. Also functions as an adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-SRSF3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: Mouse liver lysate

References

Datasheet

Anti-SRSF3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SRSF3 Polyclonal Antibody (HB340014)

Overview

Images

References

Datasheet

SDS