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Anti-STAMBP Polyclonal Antibody (HF596014)

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Overview
Catalog No.HF596014
Description
Anti-STAMBP Polyclonal Antibody (HF596014) is a rabbit polyclonal antibody detecting STAMBP in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human STAMBP (Lys126-Ser368).
Target Endosome-associated ubiquitin isopeptidase, STAM-binding protein, AMSH, Associated molecule with the SH3 domain of STAM, STAMBP
Purification Purified by antigen affinity column.
Accession O95630
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

STAM-binding protein (STAMBP) is a ~48 kDa protein. Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes.

1. McCullough, J. et al. (2004) The Journal of cell biology 166, 487-92. PMID: 15314065
2. McDonell, LM. et al. (2013) Nature genetics 45, 556-62. PMID: 23542699
3. Guo, Y. et al. (2021) The Journal of biological chemistry 297, 101107. PMID: 34425109
4. Tanaka, N. et al. (1999) The Journal of biological chemistry 274, 19129-35. PMID: 10383417
5. Itoh, F. et al. (2001) The EMBO journal 20, 4132-42. PMID: 11483516
6. Ma, YM. et al. (2007) The Journal of biological chemistry 282, 9805-9812. PMID: 17261583
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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