Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (QD20) (JN998053)

Name: Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (QD20)
Brand: abinScience
SKU: JN998053
Price: 191 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

JN998053

Species reactivity

Staphylococcus aureus

Applications

ELISA, Neutralization, SPR

Host species

Mouse

Isotype

IgG2a

Clone ID

QD20

Clonality

Monoclonal

Target

hly, hla, Alpha-hemolysin, Alpha-toxin, Alpha-HL, AT, Staphylococcus aureus, Staphylococcus aureus (strain NCTC 8325 / PS 47)

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P09616, Q2G1X0

RRID

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (QD20) (abinScience Cat# JN998053, RRID:AB_3729029)
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Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Alpha-hemolysin (Alpha-toxin/hly) is a ~35 kDa protein. Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.

1. Walker, B. et al. (1992) The Journal of biological chemistry 267, 10902-9. PMID: 1587866

2. Wilke, GA. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13473-8. PMID: 20624979

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (QD20)

References

Datasheet

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (QD20).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (QD20) (JN998053)

Overview

Images

References

Datasheet

SDS

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