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Anti-Staphylococcus aureus entD/SED Polyclonal Antibody (JN090014)

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Overview
Catalog No.JN090014
Description
Anti-Staphylococcus aureus entD/SED Polyclonal Antibody (JN090014) is a rabbit polyclonal antibody detecting entD in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityStaphylococcus aureus
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Staphylococcus aureus entD/SED (Asn26-Tyr113).
Target Enterotoxin type D, SED, entD
Purification Purified by antigen affinity column.
Accession P20723
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Enterotoxin type D (entD/SED) is a ~29 kDa protein. Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines. In addition, induces B-cell proliferation and differentiation in the presence of T-cells. Causes also the intoxication staphylococcal food poisoning syndrome.

1. Al-Daccak, R. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 225-32. PMID: 9551975
2. Domiati-Saad, R. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3608-20. PMID: 8621894
3. Kérouanton, A. et al. (2007) International journal of food microbiology 115, 369-75. PMID: 17306397
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Staphylococcus aureus entD/SED Polyclonal Antibody

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