Anti-STARD3 Polyclonal Antibody (HB422014)

Name: Anti-STARD3 Polyclonal Antibody
Brand: abinScience
SKU: HB422014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB422014

Description

Anti-STARD3 Polyclonal Antibody (HB422014) is a rabbit polyclonal antibody detecting StAR-related lipid transfer protein 3; Metastatic lymph node gene 64 protein; MLN 64; Protein CAB1; START domain-containing protein 3; StARD3; STARD3; CAB1; MLN64 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human STARD3 (Ser232-Ala445).

Target

StAR-related lipid transfer protein 3; Metastatic lymph node gene 64 protein; MLN 64; Protein CAB1; START domain-containing protein 3; StARD3; STARD3; CAB1; MLN64

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q14849, Q61542

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

StAR-related lipid transfer protein 3 (STARD3) is a ~50 kDa protein. Sterol-binding protein that mediates cholesterol transport from the endoplasmic reticulum to endosomes. The sterol transport mechanism is triggered by phosphorylation of FFAT motif that leads to membrane tethering between the endoplasmic reticulum and late endosomes via interaction with VAPA and VAPB. Acts as a lipid transfer protein that redirects sterol to the endosome at the expense of the cell membrane and favors membrane formation inside endosomes. May also mediate cholesterol transport between other membranes, such as mitochondria membrane or cell membrane. However, such results need additional experimental evidences; probably mainly mediates cholesterol transport from the endoplasmic reticulum to endosomes.

1. Alpy, F. et al. (2001) The Journal of biological chemistry 276, 4261-9. PMID: 11053434

2. Hölttä-Vuori, M. et al. (2005) Molecular biology of the cell 16, 3873-86. PMID: 15930133

3. Liapis, A. et al. (2012) PloS one 7, e34424. PMID: 22514632

4. Wilhelm, LP. et al. (2017) The EMBO journal 36, 1412-1433. PMID: 28377464

5. Di Mattia, T. et al. (2020) The EMBO journal 39, e104369. PMID: 33124732

6. Alpy, F. et al. (2013) Journal of cell science 126, 5500-12. PMID: 24105263

7. Zhang, M. et al. (2002) The Journal of biological chemistry 277, 33300-10. PMID: 12070139

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-STARD3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MCF-7 cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse lung lysate
Lane 4: Mouse heart lysate
Lane 5: Rat brain lysate
Lane 6: Rat heart lysate

References

Datasheet

Anti-STARD3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-STARD3 Polyclonal Antibody (HB422014)

Overview

Images

References

Datasheet

SDS

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