Anti-STAT1 Polyclonal Antibody (HW746014)

Name: Anti-STAT1 Polyclonal Antibody
Brand: abinScience
SKU: HW746014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HW746014

Description

Anti-STAT1 Polyclonal Antibody (HW746014) is a rabbit polyclonal antibody detecting STAT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human STAT1 (Met1-Arg683).

Target

Signal transducer and activator of transcription 1-alpha/beta, STAT1, Transcription factor ISGF-3 components p91/p84

Purification

Purified by antigen affinity column.

Accession

P42224

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Signal transducer and activator of transcription 1-alpha/beta (STAT1) is a ~87 kDa protein. Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated.

1. Rogers, RS. et al. (2003) The Journal of biological chemistry 278, 30091-7. PMID: 12764129

2. Ungureanu, D. et al. (2003) Blood 102, 3311-3. PMID: 12855578

3. DeVries, TA. et al. (2004) The Journal of biological chemistry 279, 45603-12. PMID: 15322115

4. Yeh, HM. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 3328-36. PMID: 23940278

5. Li, M. et al. (2021) Experimental cell research 407, 112784. PMID: 34508746

6. Alphonse, N. et al. (2022) Cell 185, 2354-2369.e17. PMID: 35568036

7. Liu, B. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 10626-31. PMID: 9724754

8. Chen, K. et al. (2017) Cell 170, 492-506.e14. PMID: 28753426

9. Zhang, Y. et al. (2015) Nature immunology 16, 1215-27. PMID: 26479788

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-STAT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A431 cell lysate
Lane 2: K562 cell lysate
Lane 3: PC-3 cell lysate
Lane 4: Hela cell lysate

References

Datasheet

Anti-STAT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-STAT1 Polyclonal Antibody (HW746014)

Overview

Images

References

Datasheet

SDS

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