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Anti-STAT5A Polyclonal Antibody (HW724014)

Anti-STAT5A Polyclonal Antibody
Anti-STAT5A Polyclonal Antibody
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Overview
Catalog No.HW724014
Description
Anti-STAT5A Polyclonal Antibody (HW724014) is a rabbit polyclonal antibody detecting STAT5A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Ovis aries.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat, Bos taurus, Ovis aries, Pig
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human STAT5A (Met1-Ser794).
Target STAT5A, STAT5, Signal transducer and activator of transcription 5A
Purification Purified by antigen affinity column.
Accession P42229
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Signal transducer and activator of transcription 5A (STAT5A) is a ~90 kDa protein. Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription.

Note For research use only.
Images
  • Anti-STAT5A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-STAT5A polyclonal antibody at 1ug/mL on recombinant STAT5A (Catalog No. HW724012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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