Anti-Streptococcus pyogenes EndoS Polyclonal Antibody (JN936014)

Name: Anti-Streptococcus pyogenes EndoS Polyclonal Antibody
Brand: abinScience
SKU: JN936014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

JN936014

Description

Anti-Streptococcus pyogenes EndoS Polyclonal Antibody (JN936014) is a rabbit polyclonal antibody detecting Streptococcus pyogenes EndoS in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Streptococcus pyogenes

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Streptococcus pyogenes EndoS (Glu37-Lys995).

Target

Endo-beta-N-acetylglucosaminidase

Purification

Purified by antigen affinity column.

Accession

Q99Y92

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Endo-beta-N-acetylglucosaminidase EndoS (Endo S) is a ~111 kDa protein. Endoglucosidase that acts as a host immune evasion factor by mediating hydrolysis of the N-linked glycan from the Fc region of host immunoglobulin-gamma (IgG) during infection. Specifically catalyzes the hydrolysis of the beta-1,4 linkage between the first two N-acetylglucosamine residues of the complex-type N-linked glycan located on 'Asn-297' of the Fc region of IgG antibodies (IGHG1, IGHG2, IGHG3 or IGHG4), thereby preventing interaction between IgGs and Fc receptors and ability to activate the complement pathway. Shows a specificity for biantennary complex type N-glycans; does neither cleave larger complex type glycans nor oligomannose and nor hybrid-type glycans. Specifically acts on IgGs; does not act on immunoglobulin alpha, beta, delta or mu.

1. Collin, M. et al. (2001) The EMBO journal 20, 3046-55. PMID: 11406581

2. Collin, M. et al. (2001) Infection and immunity 69, 7187-9. PMID: 11598100

3. Collin, M. et al. (2002) Infection and immunity 70, 6646-51. PMID: 12438337

4. Allhorn, M. et al. (2008) BMC microbiology 8, 3. PMID: 18182097

5. Allhorn, M. et al. (2010) Blood 115, 5080-8. PMID: 20357243

6. Sjögren, J. et al. (2011) BMC microbiology 11, 120. PMID: 21619648

7. Goodfellow, JJ. et al. (2012) Journal of the American Chemical Society 134, 8030-3. PMID: 22551167

8. Huang, W. et al. (2012) Journal of the American Chemical Society 134, 12308-18. PMID: 22747414

9. Dixon, EV. et al. (2014) The Journal of biological chemistry 289, 13876-89. PMID: 24668806

10. Trastoy, B. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 6714-9. PMID: 24753590

Note

For research use only.

Images

References

Datasheet

Anti-Streptococcus pyogenes EndoS Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Streptococcus pyogenes EndoS Polyclonal Antibody (JN936014)

Overview

Images

References

Datasheet

SDS

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