Anti-Sudan ebolavirus/SEBOV NP/Nucleoprotein Antibody (SAA1402) (VK518013)

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Datasheet

Overview

Catalog No.

VK518013

Description

Anti-Sudan ebolavirus/SEBOV NP/Nucleoprotein Antibody (SAA1402) [SAA1402] (VK518013) is a mouse monoclonal antibody detecting Nucleoprotein in ELISA, WB.

Species reactivity

Sudan ebolavirus (strain Boniface-76) (SEBOV) (Sudan Ebola virus)

Applications

ELISA, WB

Host species

Mouse

Isotype

VHH-mFc (IgG2a)

Clone ID

SAA1402

Clonality

Monoclonal

Target

Nucleoprotein, Nucleocapsid protein, Protein N, NP

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9QP77

RRID

Anti-Sudan ebolavirus/SEBOV NP/Nucleoprotein Antibody (SAA1402) (abinScience Cat# VK518013, RRID:AB_3726904)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Nucleoprotein (NP) is a ~82 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Sudan ebolavirus/SEBOV NP/Nucleoprotein Antibody (SAA1402)

References

Datasheet

Anti-Sudan ebolavirus/SEBOV NP/Nucleoprotein Antibody (SAA1402).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Sudan ebolavirus/SEBOV NP/Nucleoprotein Antibody (SAA1402) (VK518013)

Overview

Images

References

Datasheet

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