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Anti-SUMF1 Polyclonal Antibody (HV152014)

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Overview
Catalog No.HV152014
Description
Anti-SUMF1 Polyclonal Antibody (HV152014) is a rabbit polyclonal antibody detecting SUMF1 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SUMF1 (Glu113-Ser356).
Target Formylglycine-generating enzyme, FGE, SUMF1, Sulfatase-modifying factor 1, C-alpha-formylglycine-generating enzyme 1
Purification Purified by antigen affinity column.
Accession Q8NBK3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Formylglycine-generating enzyme (SUMF1) is a ~40 kDa protein. Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent. 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile. Known substrates include GALNS, ARSA, STS and ARSE.

1. Cosma, MP. et al. (2003) Cell 113, 445-56. PMID: 12757706
2. Preusser-Kunze, A. et al. (2005) The Journal of biological chemistry 280, 14900-10. PMID: 15657036
3. Dierks, T. et al. (2005) Cell 121, 541-552. PMID: 15907468
4. Roeser, D. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 81-6. PMID: 16368756
5. Schlotawa, L. et al. (2011) European journal of human genetics : EJHG 19, 253-61. PMID: 21224894
6. Holder, PG. et al. (2015) The Journal of biological chemistry 290, 15730-15745. PMID: 25931126
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-SUMF1 Polyclonal Antibody

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