Anti-SUMO2 Polyclonal Antibody (HX919014)

Name: Anti-SUMO2 Polyclonal Antibody
Brand: abinScience
SKU: HX919014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HX919014

Description

Anti-SUMO2 Polyclonal Antibody (HX919014) is a rabbit polyclonal antibody detecting SUMO2 in ELISA, IHC, WB. Suitable for Bovine, Cricetulus griseus, Danio rerio, Chicken, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SUMO2 (Met1-Tyr95).

Target

Ubiquitin-like protein SMT3B, SMT3B, SUMO-3, Smt3B, Sentrin-2, SMT3 homolog 2, SMT3H2, SUMO2, HSMT3, Small ubiquitin-related modifier 2, SUMO-2

Purification

Purified by antigen affinity column.

Accession

P61956

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Small ubiquitin-related modifier 2 (SUMO2) is a ~10 kDa protein. Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Plays a role in the regulation of sumoylation status of SETX.

1. Cappadocia, L. et al. (2015) Nature structural & molecular biology 22, 968-75. PMID: 26524494

2. Tatham, MH. et al. (2008) Nature cell biology 10, 538-46. PMID: 18408734

3. Meulmeester, E. et al. (2008) Molecular cell 30, 610-9. PMID: 18538659

4. Cong, L. et al. (2011) The Journal of biological chemistry 286, 43793-43808. PMID: 21965678

5. Kamitani, T. et al. (1998) The Journal of biological chemistry 273, 11349-53. PMID: 9556629

6. Richard, P. et al. (2013) Genes & development 27, 2227-32. PMID: 24105744

Note

For research use only.

Images

References

Datasheet

Anti-SUMO2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-SUMO2 Polyclonal Antibody (HX919014)

Overview

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References

Datasheet

SDS

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