Anti-SV40 VP1 Polyclonal Antibody (VK099014)

Name: Anti-SV40 VP1 Polyclonal Antibody
Brand: abinScience
SKU: VK099014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

VK099014

Description

Anti-SV40 VP1 Polyclonal Antibody (VK099014) is a rabbit polyclonal antibody detecting Major structural protein VP1 in ELISA, IHC, WB. Suitable for Simian virus 40.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Simian virus 40 (SV40)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant SV40 VP1 (Pro21-Gln362).

Target

Major capsid protein VP1, Major structural protein VP1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P03087

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Major capsid protein VP1 is a ~39 kDa protein. Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Binds to N-glycolylneuraminic analog of the ganglioside GM1 on the cell surface to provide virion attachment to target cell. Once attached, the virion is internalized by caveolin-mediated endocytosis and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating.

1. Neu, U. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 5219-24. PMID: 18353982

2. Schelhaas, M. et al. (2007) Cell 131, 516-29. PMID: 17981119

Note

For research use only

Images

References

Datasheet

Anti-SV40 VP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SV40 VP1 Polyclonal Antibody (VK099014)

Overview

Images

References

Datasheet

SDS

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