Anti-TAF12 Polyclonal Antibody (HS849014)

Name: Anti-TAF12 Polyclonal Antibody
Brand: abinScience
SKU: HS849014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HS849014

Description

Anti-TAF12 Polyclonal Antibody (HS849014) is a rabbit polyclonal antibody detecting TAF12 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TAF12 (Met1-Lys161).

Target

TAF2J, Transcription initiation factor TFIID subunit 12, TAFII20, TAFII20/TAFII15, TAF15, TAF12, TAFII-20/TAFII-15, Transcription initiation factor TFIID 20/15 kDa subunits

Purification

Purified by antigen affinity column.

Accession

Q16514

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription initiation factor TFIID subunit 12 (TAF12) is a ~17 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Component of the TATA-binding protein-free TAF complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473

2. Brand, M. et al. (1999) The Journal of biological chemistry 274, 18285-9. PMID: 10373431

3. Mengus, G. et al. (1995) The EMBO journal 14, 1520-31. PMID: 7729427

4. Hoffmann, A. et al. (1996) Nature 380, 356-9. PMID: 8598932

5. Hoffmann, A. et al. (1996) The Journal of biological chemistry 271, 18194-202. PMID: 8663456

6. Ogryzko, VV. et al. (1998) Cell 94, 35-44. PMID: 9674425

7. Vassilev, A. et al. (1998) Molecular cell 2, 869-75. PMID: 9885574

Note

For research use only.

Images

References

Datasheet

Anti-TAF12 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TAF12 Polyclonal Antibody (HS849014)

Overview

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References

Datasheet

SDS

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