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Anti-TAF7 Polyclonal Antibody (HB463014)

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Overview
Catalog No.HB463014
Description
Anti-TAF7 Polyclonal Antibody (HB463014) is a rabbit polyclonal antibody detecting Transcription initiation factor TFIID subunit 7; RNA polymerase II TBP-associated factor subunit F; Transcription initiation factor TFIID 55 kDa subunit; TAF(II)55; TAFII-55; TAFII55; TAF7; TAF2F; TAFII55 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human TAF7 (Met1-Ala176).
Target Transcription initiation factor TFIID subunit 7; RNA polymerase II TBP-associated factor subunit F; Transcription initiation factor TFIID 55 kDa subunit; TAF(II)55; TAFII-55; TAFII55; TAF7; TAF2F; TAFII55
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q15545, Q2HJG8, Q9R1C0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Transcription initiation factor TFIID subunit 7 (TAF7) is a ~40 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF7 forms a promoter DNA binding subcomplex of TFIID, together with TAF1 and TAF2. Part of a TFIID complex containing TAF10 (TFIID alpha) and a TFIID complex lacking TAF10 (TFIID beta).

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Wu, SY. et al. (1999) The Journal of biological chemistry 274, 23480-90. PMID: 10438527
Note For research use only
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Datasheet
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Formula
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Anti-TAF7 Polyclonal Antibody

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