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Anti-TBEV NS5 Polyclonal Antibody (VK502014)

Anti-TBEV NS5 Polyclonal Antibody
Anti-TBEV NS5 Polyclonal Antibody
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Spec:
  • 50ug
  • 100ug
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  • Datasheet

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Overview
Catalog No.VK502014
BrandabinScience
Description
Anti-TBEV NS5 Polyclonal Antibody (VK502014) is a rabbit polyclonal antibody detecting NS5 in ELISA, IHC, WB. Suitable for Langat virus and Louping ill virus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityTick-borne encephalitis virus (TBEV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Tick-borne encephalitis virus (TBEV) NS5 (Gly2512-Cys2776).
Target NS5, MRNA cap 0-1 NS5-type MT
Purification Purified by antigen affinity column.
Accession AHM02467.1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Genome polyprotein (NS5) is a ~380 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.

Note For research use only.
Images
  • Anti-TBEV NS5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NS5-LIV polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant TBEV NS5 (Catalog No: VK502012)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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