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Anti-TEC Polyclonal Antibody (HA637014)

Anti-TEC Polyclonal Antibody
Anti-TEC Polyclonal Antibody
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Overview
Catalog No.HA637014
Description
Anti-TEC Polyclonal Antibody (HA637014) is a rabbit polyclonal antibody detecting Tyrosine-protein kinase Tec; 2.7.10.2; TEC; PSCTK4 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human TEC (Glu182-Arg631).
Target Tyrosine-protein kinase Tec; 2.7.10.2; TEC; PSCTK4
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P42680, P24604
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Tyrosine-protein kinase Tec is a ~73 kDa protein. Non-receptor tyrosine kinase that contributes to signaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskeleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling.

Note For research use only
Images
  • Anti-TEC Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TEC polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse spleen lysate
    Lane 2: Rat spleen lysate
    Lane 3: Rat liver lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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