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Anti-TFDP1 Polyclonal Antibody (HC493014)

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Overview
Catalog No.HC493014
Description
Anti-TFDP1 Polyclonal Antibody (HC493014) is a rabbit polyclonal antibody detecting TFDP1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TFDP1 (Gly6-Asp410).
Target DRTF1-polypeptide 1, DRTF1, E2F dimerization partner 1, TFDP1, DP1, Transcription factor Dp-1
Purification Purified by antigen affinity column.
Accession Q14186
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Transcription factor Dp-1 (TFDP1) is a ~45 kDa protein. Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters.

1. Wu, CL. et al. (1995) Molecular and cellular biology 15, 2536-46. PMID: 7739537
2. Helin, K. et al. (1993) Genes & development 7, 1850-61. PMID: 8405995
3. Zaragoza, K. et al. (2010) Molecular and cellular biology 30, 2293-304. PMID: 20176812
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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