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Anti-TGEV RBD Polyclonal Antibody (VK727044)

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Overview
Catalog No.VK727044
Description
Anti-TGEV RBD Polyclonal Antibody (VK727044) is a rabbit polyclonal antibody detecting TGEV RBD. Suitable for Porcine transmissible gastroenteritis coronavirus, Feline coronavirus, and Porcine respiratory coronavirus.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityPorcine transmissible gastroenteritis coronavirus (TGEV), Feline coronavirus (FCoV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant TGEV RBD (Pro521-Pro667).
Target E2, Peplomer protein, S, S glycoprotein, Spike glycoprotein
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P07946
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Spike glycoprotein (RBD) is a ~160 kDa protein. S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-TGEV RBD Polyclonal Antibody

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