Anti-TK1 Polyclonal Antibody (HY157014)

Name: Anti-TK1 Polyclonal Antibody
Brand: abinScience
SKU: HY157014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HY157014

Description

Anti-TK1 Polyclonal Antibody (HY157014) is a rabbit polyclonal antibody detecting TK1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Cricetulus griseus, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TK1 (Met1-Asn234).

Target

Thymidine kinase, cytosolic, TK1

Purification

Purified by antigen affinity column.

Accession

P04183

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Thymidine kinase, cytosolic (TK1) is a ~25 kDa protein. Cell-cycle-regulated enzyme of importance in nucleotide metabolism. Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate. Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT).

1. Chang, ZF. et al. (1998) The Journal of biological chemistry 273, 12095-100. PMID: 9575153

2. Skovgaard, T. et al. (2012) The FEBS journal 279, 1777-87. PMID: 22385435

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TK1 polyclonal antibody at 1ug/mL on recombinant TK1 (Catalog No. HY157012).

References

Datasheet

Anti-TK1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TK1 Polyclonal Antibody (HY157014)

Overview

Images

References

Datasheet

SDS

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