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Anti-TKFC Polyclonal Antibody (HB538014)

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Overview
Catalog No.HB538014
Description
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human TKFC (Met1-Thr332).
Target Triokinase/FMN cyclase; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); ATP-dependent dihydroxyacetone kinase; DHA kinase; 2.7.1.28; 2.7.1.29; Glycerone kinase; Triokinase; Triose kinase; FAD-AMP lyase (cyclizing); 4.6.1.15; FAD-AMP lyase (cyclic FMN forming); FMN cyclase; TKFC; DAK
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q3LXA3, Q58DK4, F1RKQ4, Q4KLZ6, Q8VC30
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Triokinase/FMN cyclase (TKFC) is a ~58 kDa protein. Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response.

1. Diao, F. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 11706-11. PMID: 17600090
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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