Anti-TMEV VP1 Polyclonal Antibody (VK009014)

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Overview

Catalog No.

VK009014

Description

Anti-TMEV VP1 Polyclonal Antibody (VK009014) is a rabbit polyclonal antibody detecting VP1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Theiler's murine encephalomyelitis virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Theiler's murine encephalomyelitis virus VP1 (Gly647-Glu920).

Target

VP1

Purity

>90%

Purification

Purified by antigen affinity column.

Accession

P13899

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Genome polyprotein, Leader protein, L, Capsid protein VP0, VP4-VP2, Capsid protein VP4, P1A, Virion protein 4, Capsid protein VP2, P1B, Virion protein 2, Capsid protein VP3, P1C, Virion protein 3, Capsid protein VP1, P1D, Virion protein 1, Protein 2A, P2A, Protein 2B, P2B, Protein 2C, P2C, EC:3.6.4.13, Protein 3A, P3A, VPg, P3B, Protein 3B, Protease 3C, P3C, EC:3.4.22.28, Picornain 3C, RNA-directed RNA polymerase, RdRp, EC:2.7.7.48, 3D polymerase, 3Dpol, Protein 3D, 3D

Background

Genome polyprotein (VP1) is a ~256 kDa protein. Forms a complex with host RAN and probably binds to exportins carrying activated MAPK in order to mediate the hyperphosphorylation of host Phe/Gly containing nuclear pore proteins (Nups) resulting in cessation of active nucleocytoplasmic transport (Probable). Proteins with NLS signals fail to import, cellular mRNAs fail to export, and some proteins small enough for diffusion are not retained anymore (efflux). The resulting inhibition of cellular protein synthesis serves to ensure maximal viral gene expression and to evade host immune response. The leader protein also inhibits host interferon regulatory factor 3 (IRF3) dimerization, thereby blocking the transcriptional activation of IFN genes. Binds to host RNase L thereby preventing its activation by 2'-5' oligoadenylates in order to counteract the antiviral interferon-inducible OAS/RNase L pathway.

1. Ricour, C. et al. (2009) The Journal of general virology 90, 177-86. PMID: 19088287

2. Sorgeloos, F. et al. (2013) PLoS pathogens 9, e1003474. PMID: 23825954

3. Drappier, M. et al. (2018) PLoS pathogens 14, e1006989. PMID: 29652922

Note

For research use only.

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References

Datasheet