Anti-Mouse TNF-alpha Polyclonal Antibody (MF879014)

Name: Anti-Mouse TNF-alpha Polyclonal Antibody
Brand: abinScience
SKU: MF879014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MF879014

Description

Anti-TNFa/TNF-alpha Polyclonal Antibody (MF879014) is a rabbit polyclonal antibody detecting TNF-alpha in ELISA, IHC, WB. Suitable for Mouse, Rat, and Peromyscus leucopus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Mouse TNFa/TNF-alpha (Leu80-Leu235).

Tested applications

Target

C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P06804

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tumor necrosis factor (TNFa/TNF-alpha) is a ~25 kDa protein. Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. TNFa is the therapeutic target of adalimumab (Humira).

1. Ando, Y. et al. (2015) The Journal of biological chemistry 290, 5881-92. PMID: 25586176

2. Jeong, E. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 12894-12906. PMID: 32741026

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TNF polyclonal antibody at 1ug/mL on various samples.
Lane 1: PC-12 cell lysate
Lane 2: RAW264.7 cell lysate