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Anti-TPP1 Polyclonal Antibody (HT295014)

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Overview
Catalog No.HT295014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TPP1 (Met1-Val320).
Target Cell growth-inhibiting gene 1 protein, LPIC, Tripeptidyl-peptidase 1, Tripeptidyl aminopeptidase, Lysosomal pepstatin-insensitive protease, TPP1, CLN2, TPP-I, TPP-1, Tripeptidyl-peptidase I
Purification Purified by antigen affinity column.
Accession O14773
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Tripeptidyl-peptidase 1 (TPP1) is a ~61 kDa protein. Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.

1. Lin, L. et al. (2001) The Journal of biological chemistry 276, 2249-55. PMID: 11054422
2. Pal, A. et al. (2009) The Journal of biological chemistry 284, 3976-84. PMID: 19038966
3. Guhaniyogi, J. et al. (2009) The Journal of biological chemistry 284, 3985-97. PMID: 19038967
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-TPP1 Polyclonal Antibody

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