Anti-TRAF6 Polyclonal Antibody (HV564014)

Name: Anti-TRAF6 Polyclonal Antibody
Brand: abinScience
SKU: HV564014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV564014

Description

Anti-TRAF6 Polyclonal Antibody (HV564014) is a rabbit polyclonal antibody detecting TRAF6 in ELISA, IHC, WB. Suitable for Bovine, Cercocebus atys, Human, Macaca mulatta, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TRAF6 (Gln348-Asp504).

Target

RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6, Interleukin-1 signal transducer, TRAF6, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, RNF85

Purification

Purified by antigen affinity column.

Accession

Q9Y4K3

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

TNF receptor-associated factor 6 (TRAF6) is a ~59 kDa protein. E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as ECSIT, IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes.

1. Deng, L. et al. (2000) Cell 103, 351-61. PMID: 11057907

2. Conze, DB. et al. (2008) Molecular and cellular biology 28, 3538-47. PMID: 18347055

3. Yin, Q. et al. (2009) Nature structural & molecular biology 16, 658-66. PMID: 19465916

4. Yang, WL. et al. (2009) Science (New York, N.Y.) 325, 1134-8. PMID: 19713527

5. Ohtake, F. et al. (2016) Molecular cell 64, 251-266. PMID: 27746020

6. Kim, MJ. et al. (2019) Frontiers in immunology 10, 2203. PMID: 31620128

7. Xia, ZP. et al. (2009) Nature 461, 114-9. PMID: 19675569

8. Wang, Y. et al. (2006) Nature immunology 7, 139-47. PMID: 16378096

9. Lamothe, B. et al. (2007) The Journal of biological chemistry 282, 4102-12. PMID: 17135271

10. Shembade, N. et al. (2007) The EMBO journal 26, 3910-22. PMID: 17703191

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TRAF6 polyclonal antibody at 1ug/mL on recombinant TRAF6 (Catalog No. HV564012).

References

Datasheet

Anti-TRAF6 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-TRAF6 Polyclonal Antibody (HV564014)

Overview

Images

References

Datasheet

SDS

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