Anti-TRIM23 Polyclonal Antibody (HA702014)

Name: Anti-TRIM23 Polyclonal Antibody
Brand: abinScience
SKU: HA702014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HA702014

Description

Anti-TRIM23 Polyclonal Antibody (HA702014) is a rabbit polyclonal antibody detecting E3 ubiquitin-protein ligase TRIM23; 2.3.2.27; ADP-ribosylation factor domain-containing protein 1; GTP-binding protein ARD-1; RING finger protein 46; RING-type E3 ubiquitin transferase TRIM23; Tripartite motif-containing protein 23; TRIM23; ARD1; ARFD1; RNF46 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human TRIM23 (Pro388-Ala574).

Target

E3 ubiquitin-protein ligase TRIM23; 2.3.2.27; ADP-ribosylation factor domain-containing protein 1; GTP-binding protein ARD-1; RING finger protein 46; RING-type E3 ubiquitin transferase TRIM23; Tripartite motif-containing protein 23; TRIM23; ARD1; ARFD1; RNF46

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P36406, Q8BGX0, P36407

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase TRIM23 is a ~64 kDa protein. Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes.

1. Sparrer, KMJ. et al. (2017) Nature microbiology 2, 1543-1557. PMID: 28871090

Note

For research use only

Images

References

Datasheet

Anti-TRIM23 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TRIM23 Polyclonal Antibody (HA702014)

Overview

Images

References

Datasheet

SDS