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Anti-TRMT10B Polyclonal Antibody (HB371014)

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Overview
Catalog No.HB371014
Description
Anti-TRMT10B Polyclonal Antibody (HB371014) is a rabbit polyclonal antibody detecting tRNA methyltransferase 10 homolog B; 2.1.1.221; RNA (guanine-9-)-methyltransferase domain-containing protein 3; tRNA (guanine(9)-N(1))-methyltransferase TRMT10B; TRMT10B; RG9MTD3 in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human TRMT10B (Ser105-Glu316).
Target tRNA methyltransferase 10 homolog B; 2.1.1.221; RNA (guanine-9-)-methyltransferase domain-containing protein 3; tRNA (guanine(9)-N(1))-methyltransferase TRMT10B; TRMT10B; RG9MTD3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6PF06, Q08DP1, Q5RJK3, Q9D075
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

tRNA methyltransferase 10 homolog B (TRMT10B) is a ~36 kDa protein. S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs. Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs.

1. Vilardo, E. et al. (2012) Nucleic acids research 40, 11583-93. PMID: 23042678
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-TRMT10B Polyclonal Antibody

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