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Anti-TRPV5 Polyclonal Antibody (HV012014)

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Anti-TRPV5 Polyclonal Antibody
Anti-TRPV5 Polyclonal Antibody
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Overview
Catalog No.HV012014
Description
Anti-TRPV5 Polyclonal Antibody (HV012014) is a rabbit polyclonal antibody detecting TRPV5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human TRPV5 (Met1-Pro327).
Target ECaC1, Calcium transport protein 2, Epithelial calcium channel 1, TRPV5, Osm-9-like TRP channel 3, ECAC1, Transient receptor potential cation channel subfamily V member 5, OTRPC3, CaT2, ECaC, TrpV5
Purification Purified by antigen affinity column.
Accession Q9NQA5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Transient receptor potential cation channel subfamily V member 5 (TRPV5) is a ~82 kDa protein. Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. Required for normal Ca(2+) reabsorption in the kidney distal convoluted tubules. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties.

1. Peng, JB. et al. (2001) Genomics 76, 99-109. PMID: 11549322
2. Zhang, W. et al. (2008) American journal of physiology. Renal physiology 295, F1472-84. PMID: 18768590
Note For research use only.
Images
  • Anti-TRPV5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TRPV5 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Saos2 cell lysate
    Lane 4: HL-60 cell lysate
    Lane 5: Siha cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-TRPV5 Polyclonal Antibody

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