Anti-UBE2H Polyclonal Antibody (HB330014)

Name: Anti-UBE2H Polyclonal Antibody
Brand: abinScience
SKU: HB330014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB330014

Description

Anti-UBE2H Polyclonal Antibody (HB330014) is a rabbit polyclonal antibody detecting Ubiquitin-conjugating enzyme E2 H; 2.3.2.23; (E3-independent) E2 ubiquitin-conjugating enzyme H; 2.3.2.24; E2 ubiquitin-conjugating enzyme H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H; UBE2H in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human UBE2H (Met1-Leu183).

Target

Ubiquitin-conjugating enzyme E2 H; 2.3.2.23; (E3-independent) E2 ubiquitin-conjugating enzyme H; 2.3.2.24; E2 ubiquitin-conjugating enzyme H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H; UBE2H

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P62256, P62257, Q32LN1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin-conjugating enzyme E2 H (UBE2H) is a ~20 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.

1. Hoeller, D. et al. (2007) Molecular cell 26, 891-8. PMID: 17588522

2. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

3. Kaiser, P. et al. (1994) The Journal of biological chemistry 269, 8797-802. PMID: 8132613

4. Lampert, F. et al. (2018) eLife 7. PMID: 29911972

Note

For research use only

Images

References

Datasheet

Anti-UBE2H Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-UBE2H Polyclonal Antibody (HB330014)

Overview

Images

References

Datasheet

SDS