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Anti-UBE2N Polyclonal Antibody (HB321014)

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Overview
Catalog No.HB321014
Description
Anti-UBE2N Polyclonal Antibody (HB321014) is a rabbit polyclonal antibody detecting Ubiquitin-conjugating enzyme E2 N; 2.3.2.23; Bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; Ubc13; UbcH13; Ubiquitin carrier protein N; Ubiquitin-protein ligase N; UBE2N; BLU in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human UBE2N (Met1-Ile152).
Target Ubiquitin-conjugating enzyme E2 N; 2.3.2.23; Bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; Ubc13; UbcH13; Ubiquitin carrier protein N; Ubiquitin-protein ligase N; UBE2N; BLU
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P61088, Q0P5K3, P61089, Q9EQX9, Q5JXB2, Q95XX0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ubiquitin-conjugating enzyme E2 N (UBE2N) is a ~17 kDa protein. The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.

1. Shi, Y. et al. (2017) Nature communications 8, 15138. PMID: 28469175
2. Cadena, C. et al. (2019) Cell 177, 1187-1200.e16. PMID: 31006531
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-UBE2N Polyclonal Antibody

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