Anti-UCP1 Polyclonal Antibody (HB024014)

Name: Anti-UCP1 Polyclonal Antibody
Brand: abinScience
SKU: HB024014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HB024014

Description

Anti-UCP1 Polyclonal Antibody (HB024014) is a rabbit polyclonal antibody detecting UCP1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human UCP1 (Met1-Thr307).

Target

Solute carrier family 25 member 7, UCP1, SLC25A7, Mitochondrial brown fat uncoupling protein 1, Thermogenin, UCP, UCP 1

Purification

Purified by antigen affinity column.

Accession

P25874

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitochondrial brown fat uncoupling protein 1 (UCP1) is a ~33 kDa protein. Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria.

1. Hoang, T. et al. (2013) The Journal of biological chemistry 288, 36244-58. PMID: 24196960

2. Zhao, L. et al. (2017) Structure (London, England : 1993) 25, 1371-1379.e3. PMID: 28781081

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-UCP1 polyclonal antibody at 1ug/mL on recombinant UCP1 (Catalog No. HB024012).

References

Datasheet

Anti-UCP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-UCP1 Polyclonal Antibody (HB024014)

Overview

Images

References

Datasheet

SDS

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